{"id":569,"date":"2025-02-19T16:46:25","date_gmt":"2025-02-19T07:46:25","guid":{"rendered":"https:\/\/www.gxd.co.jp\/?page_id=569"},"modified":"2025-09-24T11:02:11","modified_gmt":"2025-09-24T02:02:11","slug":"ngs","status":"publish","type":"page","link":"https:\/\/www.gxd.co.jp\/?page_id=569","title":{"rendered":"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b9"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"569\" class=\"elementor elementor-569\">\n\t\t\t\t<div class=\"elementor-element elementor-element-346867d6 e-con-full e-flex e-con e-parent\" data-id=\"346867d6\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-31b401e6 elementor-widget elementor-widget-text-editor\" data-id=\"31b401e6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">1.<strong>\u80cc\u666f<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\u533b\u7642\u3078\u306e\u907a\u4f1d\u60c5\u5831\u306e\u6d3b\u7528\u304c\u9032\u307f\u3001\u4fdd\u5065\u533b\u7642\u3067\u306e\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09\u60c5\u5831\u306e\u6d3b\u7528\u3082\u884c\u308f\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u305d\u306e\u4e00\u4f8b\u3067\u3042\u308b\u304c\u3093\u30d1\u30cd\u30eb\u691c\u67fb\u3082\u7d0410\uff05\u524d\u5f8c\u306b\u3068\u3069\u307e\u3063\u3066\u304a\u308a\u3001\u7814\u7a76\u304c\u9032\u3093\u3067\u3044\u308b\u304c\u3093\u306e\u9818\u57df\u3067\u3055\u3048\u3082\u307e\u3060\u75be\u60a3\u306e\u5168\u5bb9\u89e3\u660e\u306b\u306f\u81f3\u3063\u3066\u3044\u306a\u3044\u72b6\u6cc1\u3067\u3059\u3002\u4eca\u5f8c\u3055\u3089\u306b\u3001\u75be\u60a3\u539f\u56e0\u907a\u4f1d\u5b50\u306e\u7279\u5b9a\u3084\u907a\u4f1d\u5b50\u767a\u73fe\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u6a5f\u69cb\u306e\u89e3\u660e\u304c\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">2.<strong>\u89e3\u6790\u6982\u8981\u53ca\u3073\u7279\u9577<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">GxD\u793e\u3067\u306fWGS\uff08Whole Genome Sequence\uff09\u3001eWES\uff08enrichment Whole Exome Sequence\uff09\u3001WTS\uff08Whole Transcriptome Sequence\uff09\u30b5\u30fc\u30d3\u30b9\u3092\u63d0\u4f9b\u3057\u307e\u3059<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">2-1.<strong>WGS<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\u30b2\u30ce\u30e0\u3092\u69cb\u6210\u3059\u308bDNA\u306e\u5869\u57fa\u914d\u5217\u3092\u89e3\u8aad\u3057\u307e\u3059\u3002\u75be\u60a3\u95a2\u9023\u907a\u4f1d\u5b50\u3092\u4e2d\u5fc3\u306b\u3001\u30ec\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-full wp-image-2043\" src=\"https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-rotated.jpg\" alt=\"\" width=\"2048\" height=\"676\" srcset=\"https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-rotated.jpg 2048w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-300x99.jpg 300w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-1024x338.jpg 1024w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-768x254.jpg 768w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-1-1-20250404-2-1536x507.jpg 1536w\" sizes=\"(max-width: 2048px) 100vw, 2048px\" \/><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">2-2.<strong>eWES<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\uff08formalin fixed paraffin embedded\uff1aFFPE\uff09\u691c\u4f53\u304b\u3089Twist Bioscience\u793e\u306e\u30d1\u30cd\u30eb\u3092\u7528\u3044\u3001\u5168\u907a\u4f1d\u5b50\uff08\u7d04 2 \u4e07 2 \u5343\u907a\u4f1d\u5b50\uff09\u306e\u30a8\u30af\u30bd\u30f3\u9818\u57df\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30b3\u30fc\u30c9\u3059\u308b\u90e8\u5206\uff09\u3092\u7db2\u7f85\u7684\uff08\u5305\u62ec\u7684\uff09\u306b\u89e3\u6790\u3057\u307e\u3059\u3002\u30b2\u30ce\u30e0 DNA\u4e2d\u306e\u4e00\u5869\u57fa\u5909\u7570\uff08single nucleotide variant\uff1aSNV\uff09\u3001\u633f\u5165\u6b20\u5931\u5909\u7570\uff08insertion\/deletion\uff1aINDEL\uff09\u3001\u30b3\u30d4\u30fc\u6570\u591a\u578b (copy number variation\uff1aCNV)\u306e\u691c\u51fa\u304a\u3088\u3073\u30de\u30a4\u30af\u30ed\u30b5\u30c6\u30e9\u30a4\u30c8\u4e0d\u5b89\u5b9a\u6027(microsatellite instability\uff1aMSI)\u306e\u5224\u5b9a\u3001\u816b\u760d\u907a\u4f1d\u5b50\u5909\u7570\u91cf(tumor mutational \uff42urden\uff1aTMB)\u306e\u7b97\u51fa\u3092\u884c\u3044\u307e\u3059\u3002\u307e\u305f\u4e00\u90e8\u306e\u907a\u4f1d\u5b50\u306b\u3064\u3044\u3066\u306f\u30a8\u30f3\u30ea\u30c3\u30c1\u30e1\u30f3\u30c8\u3092\u884c\u3063\u305f\u5f8c\u306bDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3044\u307e\u3059\u3002WES\u306fWGS\u89e3\u6790\u306e\u88dc\u5b8c\u7684\u306a\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u3082\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">2-3.<strong>WTS<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\uff08formalin fixed paraffin embedded\uff1aFFPE\uff09\u691c\u4f53\u304b\u3089Twist Bioscience\u793e\u306e\u30d1\u30cd\u30eb\u3092\u7528\u3044\u3001\u5869\u57fa35.8 Mb\u3001\u907a\u4f1d\u5b5019,708\u500b\u304a\u3088\u3073\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e063,215\u500b\u3092\u89e3\u6790\u3057\u3001\u878d\u5408\u907a\u4f1d\u5b50\uff08fusion\uff09\u3001\u30a8\u30af\u30bd\u30f3\u30b9\u30ad\u30c3\u30d4\u30f3\u30b0\uff08exon skipping\uff09\u306e\u691c\u51fa\u3092\u884c\u3044\u307e\u3059\u3002<\/span><\/p>\n<p style=\"text-align: left;\">\u00a0<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">3.<strong>\u30aa\u30d7\u30b7\u30e7\u30f3<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">Spatial Transcriptomics\u89e3\u6790\u3084MRD\u89e3\u6790\u3001Proteomics\u89e3\u6790\u3068\u4f75\u305b\u3066\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u306e\u3067\u3001\u5b9f\u9a13\u76ee\u7684\u306b\u5fdc\u3058\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/span><\/p>\n<p style=\"text-align: left;\">\u00a0<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">4.<strong>\u691c\u4f53\u6761\u4ef6<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\" data-teams=\"true\">\u30fbWGS\uff1a\u982c\u7c98\u819c\u304b\u8840\u6db2\u691c\u4f53<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\u30fbeWES\u30fbWTS\uff1aFFPE<\/span><\/p>\n<p style=\"text-align: left;\">\u00a0<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">5.<strong>\u7d0d\u54c1\u7269<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">eWES \u30fbWGS (Fastq\u30d5\u30a1\u30a4\u30eb\u3001VCF\u30d5\u30a1\u30a4\u30eb\u3001BAM\u30d5\u30a1\u30a4\u30eb)<\/span><br \/><span style=\"color: #333333;\">WTS (Fastq\u30d5\u30a1\u30a4\u30eb\u3001 TPM\u3082\u3057\u304f\u306fFPKM\u306b\u3088\u308b\u30ab\u30a6\u30f3\u30c8\u5024\u30c7\u30fc\u30bf)<\/span><\/p>\n<p style=\"text-align: left;\">\u00a0<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<h5 class=\"wp-block-heading\" style=\"text-align: left;\"><span style=\"color: #333333;\">6.<strong>\u76ee\u5b89\u7d0d\u671f\u53ca\u3073\u4fa1\u683c<\/strong><\/span><\/h5>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\">\uff14\u9031\u9593\uff08\u30b5\u30f3\u30d7\u30eb\u306b\u3088\u3063\u3066\u7d0d\u671f\u3068\u4fa1\u683c\u304c\u5909\u308f\u308a\u307e\u3059\u306e\u3067\u3001\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\uff09<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><img decoding=\"async\" class=\"alignnone size-full wp-image-2029\" src=\"https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404.png\" alt=\"\" width=\"2708\" height=\"1844\" srcset=\"https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404.png 2708w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404-300x204.png 300w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404-1024x697.png 1024w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404-768x523.png 768w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404-1536x1046.png 1536w, https:\/\/www.gxd.co.jp\/wp-content\/uploads\/2025\/04\/NGS-20250404-2048x1395.png 2048w\" sizes=\"(max-width: 2708px) 100vw, 2708px\" \/><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"color: #333333;\">\u25a0Provided by <span style=\"color: #333399;\"><strong><a style=\"color: #333399;\" href=\"https:\/\/jp.illumina.com\/\" target=\"_blank\" rel=\"noopener\">illumina<\/a><\/strong><\/span><\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"color: #333333;\"><\/span><\/p>\n<div class=\"stephow-hover-overlay\" style=\"opacity: 0; text-align: left;\">\u00a0<\/div>\n<div id=\"guideflo-extension-unique-identifier\" style=\"display: none; text-align: left;\">\u00a0<\/div>\n<div class=\"stephow-hover-overlay\" style=\"opacity: 0; text-align: left;\">\u00a0<\/div>\n<div id=\"guideflo-extension-unique-identifier\" style=\"display: none; text-align: left;\">\u00a0<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>1.\u80cc\u666f \u533b\u7642\u3078\u306e\u907a\u4f1d\u60c5\u5831\u306e\u6d3b\u7528\u304c\u9032\u307f\u3001\u4fdd\u5065\u533b\u7642\u3067\u306e\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09\u60c5\u5831\u306e\u6d3b\u7528\u3082\u884c\u308f\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u305d\u306e\u4e00\u4f8b\u3067\u3042\u308b\u304c\u3093\u30d1\u30cd\u30eb\u691c\u67fb\u3082\u7d0410\uff05\u524d\u5f8c\u306b\u3068\u3069\u307e\u3063\u3066\u304a\u308a\u3001\u7814\u7a76\u304c\u9032\u3093\u3067\u3044\u308b\u304c\u3093\u306e\u9818\u57df\u3067\u3055\u3048 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":15,"comment_status":"closed","ping_status":"closed","template":"pagetemplates\/fullwidth-page.php","meta":{"footnotes":""},"class_list":["post-569","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/pages\/569","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=569"}],"version-history":[{"count":62,"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/pages\/569\/revisions"}],"predecessor-version":[{"id":2588,"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=\/wp\/v2\/pages\/569\/revisions\/2588"}],"wp:attachment":[{"href":"https:\/\/www.gxd.co.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=569"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}